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KMID : 0364820120480040328
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Korean Journal of Microbiology
2012 Volume.48 No. 4 p.328 ~ p.331
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Marine Prokaryotic Diversity of the Deep Sea Waters at the Depth of 1500 m Off the Coast of the Ulleung Island in the East Sea (Korea)
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Kim Mi-Kyung
Khang Yong-Ho
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Abstract
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Microbial diversity in the 1500 m depth sea waters off the coast of Ulleung island of the East Sea, Korea, was
investigated. Genomic DNAs were extracted directly from the marine microbes filtered through ultramembrane
filters. Pyrosequencing of 16S rDNAs of these microbes resulted in 13,029 reads, of which uncultured bacteria
consisted of 54.1%, alphaproteobacteria 23.4%, and gammaproteobacteria 22.3%. Other classes such as
flavobacteria, actinobacteria, and epsilonproteobacteria were distributed within 0.2% of total reads. Among the
cultivable bacteria, it was found that Rhodobacteraceae family of alphaproteobacteria, Alteromonadaceae,
Halomonadaceae, and Piscirickettsiaceae families of gammaproteobacteria were mostly distributed in the deep-sea
waters
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KEYWORD
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deep seawater, East Sea, marine microbe, pyrosequencing, Ulleng Island
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